產品特點:
1.適合細胞或細胞簇的定位和重找回;
2.ibidi標準底部設計,180um的蓋玻片尺寸底部厚度,適合高分辨率顯微觀察;
3.ibiTreat 底部包被,適合大多數細胞的貼壁培養;
4.旋轉蓋部設計減小液體揮發。
應用:
1.細胞或細胞簇的再定位
2.細胞計數,例如:細胞轉染效率計算
3.細胞運動觀察
4.細胞顯微操作中單細胞處理
技術特征:
1.直徑為35mm標準樣式的培養皿;
2.具500µm 網格刻度;
3.字母或數字標記(A-U; 1-20);
4.替代選擇的玻璃底部產品:µ-Slide 35 mm, high glass bottom with Grid-50 or with Grid-500;
5.ibiTreat 組織培養處理表面適合大多數細胞的貼壁生長;
6.生物相容性材料制作,不含膠水,無細胞毒性影響;
7.旋轉蓋部設計,減小液體揮發。
產品規格:
Grid repeat distance
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500 µm |
Ø µ-Dish |
35 mm |
Volume high / low |
2 / 0.8 ml |
Growth area |
3.5 cm2 |
Ø observation area |
21 mm |
Height with lid high / low |
14 / 9 mm |
Bottom: ibidi Standard Bottom |
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Layout Grid-500
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Lid with Lock Position
The special lock feature in all ibidi µ-Dishes enables the user to minimize evaporation. The lock position provides excellent conditions for long term studies in non humidified environments. Gas exchange (carbon dioxide, oxygen) during cell culture is maintained thanks to the gas permeable plastic material of the dish.
TIP: Use the locking feature only if minimal evaporation is
required, i.e., outside incubators, non humidified microscopy stages, etc.
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click here to enlarge
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ibidi µ-Dish Family: Versions and Variations
µ-Dish 35 mm, high
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µ-Dish 35 mm, low |
µ-Dish 50 mm, low |
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High walls:
標準應用
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Low walls:
細胞顯微操作
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ibidi Standard Bottom vs. ibidi Glass Bottom
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ibidi Standard Bottom
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ibidi Glass Bottom
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Optical properties |
Refractive index (nD 589 nm) |
1.52 |
1.52 |
Abbe number |
56 |
55 |
Thickness |
#1.5 (180 µm) |
#1.5 (170 µm) |
Material |
Microscopy plastic |
D 263M Schott borosilicate glass |
Autofluorescence |
Low |
Low |
Transmission |
Very high (even ultraviolet) |
High (ultraviolet restrictions) |
Birefringence (DIC) |
Low (DIC compatible*) |
Low (DIC compatible*) |
Other aspects |
Surface modifications |
ibiTreat – tissue culture treated
Uncoated – hydrophobic |
Only pure glass |
Protein coatings |
Possible |
Possible |
Gas permeable |
Yes |
No |
Material flexibility |
High |
Low |
Breakable |
No |
Yes |
Recommended for |
All kinds of fluorescence microscopy |
TIRF and single photon |
* Special glass DIC Lids are available separately
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訂購信息:
貨號 |
產品名稱 |
規格(個/盒) |
81148 |
µ-Dish 35 mm,高壁,玻璃底,50微米格子培養皿 |
30 |
81168 |
µ-Dish 35 mm,高壁,玻璃底,500微米格子培養皿 |
30 |
80156 |
µ-Dish 35 mm,低壁,ibiTreat底部處理,500微米格子培養皿 |
60 |
80151 |
µ-Dish 35 mm,低壁,無包被,500微米格子培養皿 |
60 |
81166 |
µ-Dish 35 mm,高壁,ibiTreat底部處理,500微米格子培養皿 |
60 |
81161 |
µ-Dish 35 mm,高壁,無包被,500微米格子培養皿 |
60 |
Cat. No. |
Description |
Pcs./Box |
80156
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µ-Dish 35 mm, low Grid-500, ibiTreat, tissue culture treated, sterile, low wall, Ø 35 mm, grid repeat distance 500 µm
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60
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80151
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µ-Dish 35 mm, low Grid-500, hydrophobic, uncoated, sterile, low wall, Ø 35 mm, grid repeat distance 500 µm
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60
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81166
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µ-Dish 35 mm, high Grid-500, ibiTreat, tissue culture treated, sterile, high wall, Ø 35 mm, 2 ml volume, grid repeat distance 500 µm
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60
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81161
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µ-Dish 35 mm, high Grid-500, hydrophobic, uncoated, sterile, high wall, Ø 35 mm, 2 ml volume, grid repeat distance 500 µm
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60
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